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tbe buffer  (Thermo Fisher)
96
Thermo Fisher tbe buffer
Tbe Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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novex tbe urea sample buffer  (Thermo Fisher)
96
Thermo Fisher novex tbe urea sample buffer
Analysis of tDR production in response to mitochondrial stress. A: MTT cytotoxicity assay after exposure to different stressors for 4 h. Asterisk indicates statistical significance compared to control (i.e. untreated) cells. B: Puromycin incorporation assay 4 h after stress induction. C: Western blot analysis of P38 and p-P38 4 h after stress induction. D: Western blot analysis of eIF2α and p -eIF2α 4 h after stress induction. E: Western blot analysis of P70S6 K and p-P70S6 K 4 h after stress induction. F: SYBR gold staining <t>of</t> <t>TBE-Urea</t> gels (long exposure) showing the production of tDRs after different stresses . 5s rRNA band shown is cropped from the same membrane at shorter exposure time. G: Sequencing strategy for the detection of tDRs production after mitochondrial stress. Cells were exposed to Rotenone (10 μM), Antimycin A (10 μg/mL) or Arsenite (100 μM) for 8 h then small RNAs extracted and sequenced. Three biological replicates were sequenced per group, except control where 2 biological replicates were sequenced. H-J: Volcano plots showing the differentially expressed tDRs in each condition versus the control group. K: PLS-DA clustering analysis revealing the differential clustering patterns observed in each stress condition. Abbreviations: CO: control; RO: Rotenone; TTFA; Thenoyltrifluoroacetone; AM: Antimycin A; KCN: Potassium Cyanide; OLI: Oligomycin; AS: Arsenite.
Novex Tbe Urea Sample Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
novex tbe urea sample buffer - by Bioz Stars, 2026-03
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buffer  (Thermo Fisher)
96
Thermo Fisher buffer
Analysis of tDR production in response to mitochondrial stress. A: MTT cytotoxicity assay after exposure to different stressors for 4 h. Asterisk indicates statistical significance compared to control (i.e. untreated) cells. B: Puromycin incorporation assay 4 h after stress induction. C: Western blot analysis of P38 and p-P38 4 h after stress induction. D: Western blot analysis of eIF2α and p -eIF2α 4 h after stress induction. E: Western blot analysis of P70S6 K and p-P70S6 K 4 h after stress induction. F: SYBR gold staining <t>of</t> <t>TBE-Urea</t> gels (long exposure) showing the production of tDRs after different stresses . 5s rRNA band shown is cropped from the same membrane at shorter exposure time. G: Sequencing strategy for the detection of tDRs production after mitochondrial stress. Cells were exposed to Rotenone (10 μM), Antimycin A (10 μg/mL) or Arsenite (100 μM) for 8 h then small RNAs extracted and sequenced. Three biological replicates were sequenced per group, except control where 2 biological replicates were sequenced. H-J: Volcano plots showing the differentially expressed tDRs in each condition versus the control group. K: PLS-DA clustering analysis revealing the differential clustering patterns observed in each stress condition. Abbreviations: CO: control; RO: Rotenone; TTFA; Thenoyltrifluoroacetone; AM: Antimycin A; KCN: Potassium Cyanide; OLI: Oligomycin; AS: Arsenite.
Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/buffer/product/Thermo Fisher
Average 96 stars, based on 1 article reviews
buffer - by Bioz Stars, 2026-03
96/100 stars
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novex 2x tbe urea sample buffer  (Thermo Fisher)
96
Thermo Fisher novex 2x tbe urea sample buffer
Analysis of tDR production in response to mitochondrial stress. A: MTT cytotoxicity assay after exposure to different stressors for 4 h. Asterisk indicates statistical significance compared to control (i.e. untreated) cells. B: Puromycin incorporation assay 4 h after stress induction. C: Western blot analysis of P38 and p-P38 4 h after stress induction. D: Western blot analysis of eIF2α and p -eIF2α 4 h after stress induction. E: Western blot analysis of P70S6 K and p-P70S6 K 4 h after stress induction. F: SYBR gold staining <t>of</t> <t>TBE-Urea</t> gels (long exposure) showing the production of tDRs after different stresses . 5s rRNA band shown is cropped from the same membrane at shorter exposure time. G: Sequencing strategy for the detection of tDRs production after mitochondrial stress. Cells were exposed to Rotenone (10 μM), Antimycin A (10 μg/mL) or Arsenite (100 μM) for 8 h then small RNAs extracted and sequenced. Three biological replicates were sequenced per group, except control where 2 biological replicates were sequenced. H-J: Volcano plots showing the differentially expressed tDRs in each condition versus the control group. K: PLS-DA clustering analysis revealing the differential clustering patterns observed in each stress condition. Abbreviations: CO: control; RO: Rotenone; TTFA; Thenoyltrifluoroacetone; AM: Antimycin A; KCN: Potassium Cyanide; OLI: Oligomycin; AS: Arsenite.
Novex 2x Tbe Urea Sample Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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novex tbe urea  (Thermo Fisher)
96
Thermo Fisher novex tbe urea
Analysis of tDR production in response to mitochondrial stress. A: MTT cytotoxicity assay after exposure to different stressors for 4 h. Asterisk indicates statistical significance compared to control (i.e. untreated) cells. B: Puromycin incorporation assay 4 h after stress induction. C: Western blot analysis of P38 and p-P38 4 h after stress induction. D: Western blot analysis of eIF2α and p -eIF2α 4 h after stress induction. E: Western blot analysis of P70S6 K and p-P70S6 K 4 h after stress induction. F: SYBR gold staining <t>of</t> <t>TBE-Urea</t> gels (long exposure) showing the production of tDRs after different stresses . 5s rRNA band shown is cropped from the same membrane at shorter exposure time. G: Sequencing strategy for the detection of tDRs production after mitochondrial stress. Cells were exposed to Rotenone (10 μM), Antimycin A (10 μg/mL) or Arsenite (100 μM) for 8 h then small RNAs extracted and sequenced. Three biological replicates were sequenced per group, except control where 2 biological replicates were sequenced. H-J: Volcano plots showing the differentially expressed tDRs in each condition versus the control group. K: PLS-DA clustering analysis revealing the differential clustering patterns observed in each stress condition. Abbreviations: CO: control; RO: Rotenone; TTFA; Thenoyltrifluoroacetone; AM: Antimycin A; KCN: Potassium Cyanide; OLI: Oligomycin; AS: Arsenite.
Novex Tbe Urea, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
novex tbe urea - by Bioz Stars, 2026-03
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1x tris borate edta tbe buffer  (Thermo Fisher)
99
Thermo Fisher 1x tris borate edta tbe buffer
Analysis of tDR production in response to mitochondrial stress. A: MTT cytotoxicity assay after exposure to different stressors for 4 h. Asterisk indicates statistical significance compared to control (i.e. untreated) cells. B: Puromycin incorporation assay 4 h after stress induction. C: Western blot analysis of P38 and p-P38 4 h after stress induction. D: Western blot analysis of eIF2α and p -eIF2α 4 h after stress induction. E: Western blot analysis of P70S6 K and p-P70S6 K 4 h after stress induction. F: SYBR gold staining <t>of</t> <t>TBE-Urea</t> gels (long exposure) showing the production of tDRs after different stresses . 5s rRNA band shown is cropped from the same membrane at shorter exposure time. G: Sequencing strategy for the detection of tDRs production after mitochondrial stress. Cells were exposed to Rotenone (10 μM), Antimycin A (10 μg/mL) or Arsenite (100 μM) for 8 h then small RNAs extracted and sequenced. Three biological replicates were sequenced per group, except control where 2 biological replicates were sequenced. H-J: Volcano plots showing the differentially expressed tDRs in each condition versus the control group. K: PLS-DA clustering analysis revealing the differential clustering patterns observed in each stress condition. Abbreviations: CO: control; RO: Rotenone; TTFA; Thenoyltrifluoroacetone; AM: Antimycin A; KCN: Potassium Cyanide; OLI: Oligomycin; AS: Arsenite.
1x Tris Borate Edta Tbe Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x tbe buffer  (Thermo Fisher)
96
Thermo Fisher 1x tbe buffer
Analysis of tDR production in response to mitochondrial stress. A: MTT cytotoxicity assay after exposure to different stressors for 4 h. Asterisk indicates statistical significance compared to control (i.e. untreated) cells. B: Puromycin incorporation assay 4 h after stress induction. C: Western blot analysis of P38 and p-P38 4 h after stress induction. D: Western blot analysis of eIF2α and p -eIF2α 4 h after stress induction. E: Western blot analysis of P70S6 K and p-P70S6 K 4 h after stress induction. F: SYBR gold staining <t>of</t> <t>TBE-Urea</t> gels (long exposure) showing the production of tDRs after different stresses . 5s rRNA band shown is cropped from the same membrane at shorter exposure time. G: Sequencing strategy for the detection of tDRs production after mitochondrial stress. Cells were exposed to Rotenone (10 μM), Antimycin A (10 μg/mL) or Arsenite (100 μM) for 8 h then small RNAs extracted and sequenced. Three biological replicates were sequenced per group, except control where 2 biological replicates were sequenced. H-J: Volcano plots showing the differentially expressed tDRs in each condition versus the control group. K: PLS-DA clustering analysis revealing the differential clustering patterns observed in each stress condition. Abbreviations: CO: control; RO: Rotenone; TTFA; Thenoyltrifluoroacetone; AM: Antimycin A; KCN: Potassium Cyanide; OLI: Oligomycin; AS: Arsenite.
1x Tbe Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1x tbe buffer/product/Thermo Fisher
Average 96 stars, based on 1 article reviews
1x tbe buffer - by Bioz Stars, 2026-03
96/100 stars
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Analysis of tDR production in response to mitochondrial stress. A: MTT cytotoxicity assay after exposure to different stressors for 4 h. Asterisk indicates statistical significance compared to control (i.e. untreated) cells. B: Puromycin incorporation assay 4 h after stress induction. C: Western blot analysis of P38 and p-P38 4 h after stress induction. D: Western blot analysis of eIF2α and p -eIF2α 4 h after stress induction. E: Western blot analysis of P70S6 K and p-P70S6 K 4 h after stress induction. F: SYBR gold staining of TBE-Urea gels (long exposure) showing the production of tDRs after different stresses . 5s rRNA band shown is cropped from the same membrane at shorter exposure time. G: Sequencing strategy for the detection of tDRs production after mitochondrial stress. Cells were exposed to Rotenone (10 μM), Antimycin A (10 μg/mL) or Arsenite (100 μM) for 8 h then small RNAs extracted and sequenced. Three biological replicates were sequenced per group, except control where 2 biological replicates were sequenced. H-J: Volcano plots showing the differentially expressed tDRs in each condition versus the control group. K: PLS-DA clustering analysis revealing the differential clustering patterns observed in each stress condition. Abbreviations: CO: control; RO: Rotenone; TTFA; Thenoyltrifluoroacetone; AM: Antimycin A; KCN: Potassium Cyanide; OLI: Oligomycin; AS: Arsenite.

Journal: Redox Biology

Article Title: Context-specific Angiogenin-mediated tRNA fragments (tDRs) biogenesis shapes the mitochondrial stress response

doi: 10.1016/j.redox.2026.104038

Figure Lengend Snippet: Analysis of tDR production in response to mitochondrial stress. A: MTT cytotoxicity assay after exposure to different stressors for 4 h. Asterisk indicates statistical significance compared to control (i.e. untreated) cells. B: Puromycin incorporation assay 4 h after stress induction. C: Western blot analysis of P38 and p-P38 4 h after stress induction. D: Western blot analysis of eIF2α and p -eIF2α 4 h after stress induction. E: Western blot analysis of P70S6 K and p-P70S6 K 4 h after stress induction. F: SYBR gold staining of TBE-Urea gels (long exposure) showing the production of tDRs after different stresses . 5s rRNA band shown is cropped from the same membrane at shorter exposure time. G: Sequencing strategy for the detection of tDRs production after mitochondrial stress. Cells were exposed to Rotenone (10 μM), Antimycin A (10 μg/mL) or Arsenite (100 μM) for 8 h then small RNAs extracted and sequenced. Three biological replicates were sequenced per group, except control where 2 biological replicates were sequenced. H-J: Volcano plots showing the differentially expressed tDRs in each condition versus the control group. K: PLS-DA clustering analysis revealing the differential clustering patterns observed in each stress condition. Abbreviations: CO: control; RO: Rotenone; TTFA; Thenoyltrifluoroacetone; AM: Antimycin A; KCN: Potassium Cyanide; OLI: Oligomycin; AS: Arsenite.

Article Snippet: In brief, 3 μg of total RNA were mixed with Novex TBE-Urea Sample Buffer (Thermo Fisher Scientific, Cat# LC6876), heated at 70 °C for 3 min, and loaded onto Novex 10 % TBE-Urea gels (Invitrogen, Cat# EC68755BOX) in 0.5 × TBE buffer (Bio-Rad, Cat# 1610733).

Techniques: Cytotoxicity Assay, Control, Western Blot, Staining, Membrane, Sequencing